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Malignant cells should be sent for conventional cytogenetic studies, as detection of the Ph1 t(9;22), gene rearrangements add important prognostic information.

Flow cytometry should be performed to characterize expression of lineage-defining antigens and allow determination of the specific ALL subtype.

Long-term follow-up of 30 patients with ALL in remission for at least 10 years has demonstrated ten cases of secondary malignancies.

Of 31 long-term female survivors of ALL or AML younger than 40 years, 26 resumed normal menstruation following completion of therapy.

Many patients who have molecular evidence of the hybridization (FISH) or reverse-transcriptase polymerase chain reaction (RT-PCR) because many patients have a different fusion protein from the one found in CML (p190 vs. These tests should be performed, whenever possible, in patients with ALL, especially in those with B-cell lineage disease.

L3 ALL is associated with a variety of translocations that involve translocation of the A bone marrow biopsy and aspirate are routinely performed even in T-cell ALL to determine the extent of marrow involvement.

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